H2N2V1, Main, Exploration, bibRecord, 001987

Structure of influenza haemagglutinin at neutral and at fusogenic pH by electron cryo-microscopy

Identifieur interne : 001987 ( Main/Exploration ); précédent : 001986; suivant : 001988

Structure of influenza haemagglutinin at neutral and at fusogenic pH by electron cryo-microscopy

Auteurs : Christoph Böttcher [Allemagne] ; Kai Ludwig [Allemagne] ; Andreas Herrmann [Allemagne] ; Marin Van Heel [Royaume-Uni] ; Holger Stark [Allemagne]

Source :

FEBS Letters [ 0014-5793 ] ; 1999.

RBID : ISTEX:2E44183DE2006E8235FC42EB2D04C37014438D4A

Descripteurs français

KwdFr :
- Concentration en ions d'hydrogène, Conformation des protéines, Cryomicroscopie électronique, Hémagglutinines virales (), Hémagglutinines virales (ultrastructure), Modèles moléculaires, Virus de la grippe A ().
MESH :
- ultrastructure : Hémagglutinines virales.
- Concentration en ions d'hydrogène, Conformation des protéines, Cryomicroscopie électronique, Hémagglutinines virales, Modèles moléculaires, Virus de la grippe A.

English descriptors

KwdEn :
- Cryoelectron Microscopy, Hemagglutinins, Viral (chemistry), Hemagglutinins, Viral (ultrastructure), Hydrogen-Ion Concentration, Influenza A virus (chemistry), Models, Molecular, Protein Conformation.
MESH :
- chemical , chemistry : Hemagglutinins, Viral.
- chemical , ultrastructure : Hemagglutinins, Viral.
- chemistry : Influenza A virus.
Teeft :
- Acidic, Angular approach, Angular reconstitution, Biol, Cell biol, Central cavity, Cold spring harb, Conformation, Conformational, Conformational change, Conformational states, Conformational transitions, Cryoelectron Microscopy, Crystallographic structure, Digitised micrographs, Distal domains, Ectodomain, Entire ectodomain, Feb, Fourier shell correlation, Fourier space, Fusion capacity, Fusion peptide, Fusion sequence, Geel zwart, Haemagglutinin, Haemagglutinin trimer, Hydrogen-Ion Concentration, Image processing procedure, Individual trimers, Loop region, Membrane, Micrographs, Models, Molecular, Projection images, Protein Conformation, Rosette, Skehel, Subunit, Target membrane, Target membranes, Trimer, Trimeric ectodomain, Viral, Viral membrane, Whole trimer.

Abstract

Abstract: The three-dimensional structures of the complete haemagglutinin (HA) of influenza virus A/Japan/305/57 (H2N2) in its native (neutral pH) and membrane fusion-competent (low pH) form by electron cryo-microscopy at a resolution of 10 Å and 14 Å, respectively, have been determined. In the fusion-competent form the subunits remain closely associated preserving typical overall features of the trimeric ectodomain at neutral pH. Rearrangements of the tertiary structure in the distal and the stem parts are associated with the formation of a central cavity through the entire ectodomain. We suggest that the cavity is essential for relocation of the so-called fusion sequence of HA towards the target membrane.

Url:

https://api.istex.fr/ark:/67375/6H6-PCN76582-4/fulltext.pdf

DOI: 10.1016/S0014-5793(99)01475-1

Affiliations:

Le document en format XML

<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>Structure of influenza haemagglutinin at neutral and at fusogenic pH by electron cryo-microscopy</title>
<author><name sortKey="Bottcher, Christoph" sort="Bottcher, Christoph" uniqKey="Bottcher C" first="Christoph" last="Böttcher">Christoph Böttcher</name>
</author>
<author><name sortKey="Ludwig, Kai" sort="Ludwig, Kai" uniqKey="Ludwig K" first="Kai" last="Ludwig">Kai Ludwig</name>
</author>
<author><name sortKey="Herrmann, Andreas" sort="Herrmann, Andreas" uniqKey="Herrmann A" first="Andreas" last="Herrmann">Andreas Herrmann</name>
</author>
<author><name sortKey="Van Heel, Marin" sort="Van Heel, Marin" uniqKey="Van Heel M" first="Marin" last="Van Heel">Marin Van Heel</name>
</author>
<author><name sortKey="Stark, Holger" sort="Stark, Holger" uniqKey="Stark H" first="Holger" last="Stark">Holger Stark</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:2E44183DE2006E8235FC42EB2D04C37014438D4A</idno>
<date when="1999" year="1999">1999</date>
<idno type="doi">10.1016/S0014-5793(99)01475-1</idno>
<idno type="url">https://api.istex.fr/ark:/67375/6H6-PCN76582-4/fulltext.pdf</idno>
<idno type="wicri:Area/Istex/Corpus">000220</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000220</idno>
<idno type="wicri:Area/Istex/Curation">000220</idno>
<idno type="wicri:Area/Istex/Checkpoint">000787</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000787</idno>
<idno type="wicri:doubleKey">0014-5793:1999:Bottcher C:structure:of:influenza</idno>
<idno type="wicri:Area/Main/Merge">001A34</idno>
<idno type="wicri:source">PubMed</idno>
<idno type="RBID">pubmed:10606732</idno>
<idno type="wicri:Area/PubMed/Corpus">000372</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000372</idno>
<idno type="wicri:Area/PubMed/Curation">000372</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000372</idno>
<idno type="wicri:Area/PubMed/Checkpoint">000336</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000336</idno>
<idno type="wicri:Area/Ncbi/Merge">000012</idno>
<idno type="wicri:Area/Ncbi/Curation">000012</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">000012</idno>
<idno type="wicri:doubleKey">0014-5793:1999:Bottcher C:structure:of:influenza</idno>
<idno type="wicri:Area/Main/Merge">001A14</idno>
<idno type="wicri:Area/Main/Curation">001987</idno>
<idno type="wicri:Area/Main/Exploration">001987</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title level="a">Structure of influenza haemagglutinin at neutral and at fusogenic pH by electron cryo-microscopy</title>
<author><name sortKey="Bottcher, Christoph" sort="Bottcher, Christoph" uniqKey="Bottcher C" first="Christoph" last="Böttcher">Christoph Böttcher</name>
<affiliation wicri:level="3"><country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Freie Universität Berlin, Institut für Chemie/Forschungszentrum für Elektronenmikroskopie, Fabeckstr. 36a, D-14195 Berlin</wicri:regionArea>
<placeName><region type="land" nuts="3">Berlin</region>
<settlement type="city">Berlin</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Ludwig, Kai" sort="Ludwig, Kai" uniqKey="Ludwig K" first="Kai" last="Ludwig">Kai Ludwig</name>
<affiliation wicri:level="3"><country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Humboldt-Universität zu Berlin, Math.-Nat. Fak. I, Institut für Biologie/Biophysik, Invalidenstr. 43, D-10115 Berlin</wicri:regionArea>
<placeName><region type="land" nuts="3">Berlin</region>
<settlement type="city">Berlin</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Herrmann, Andreas" sort="Herrmann, Andreas" uniqKey="Herrmann A" first="Andreas" last="Herrmann">Andreas Herrmann</name>
<affiliation wicri:level="3"><country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Humboldt-Universität zu Berlin, Math.-Nat. Fak. I, Institut für Biologie/Biophysik, Invalidenstr. 43, D-10115 Berlin</wicri:regionArea>
<placeName><region type="land" nuts="3">Berlin</region>
<settlement type="city">Berlin</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Van Heel, Marin" sort="Van Heel, Marin" uniqKey="Van Heel M" first="Marin" last="Van Heel">Marin Van Heel</name>
<affiliation wicri:level="1"><country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>Imperial College of Science, Medicine and Technology, Wolfson Laboratories, Department of Biochemistry, London SW7 2AY</wicri:regionArea>
<wicri:noRegion>London SW7 2AY</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Stark, Holger" sort="Stark, Holger" uniqKey="Stark H" first="Holger" last="Stark">Holger Stark</name>
<affiliation wicri:level="3"><country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Fritz-Haber-Institut, Max-Planck-Gesellschaft, Faradayweg 4-6, D-14195 Berlin</wicri:regionArea>
<placeName><region type="land" nuts="3">Berlin</region>
<settlement type="city">Berlin</settlement>
</placeName>
</affiliation>
<affiliation wicri:level="3"><country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Institut für Molekularbiologie und Tumorforschung, Emil-Mannkopff-Strasse 2, D-35037 Marburg</wicri:regionArea>
<placeName><region type="land" nuts="1">Hesse (Land)</region>
<region type="district" nuts="2">District de Giessen</region>
<settlement type="city">Marbourg</settlement>
</placeName>
</affiliation>
<affiliation></affiliation>
<affiliation wicri:level="1"><country wicri:rule="url">Allemagne</country>
</affiliation>
</author>
</analytic>
<monogr></monogr>
<series><title level="j">FEBS Letters</title>
<title level="j" type="abbrev">FEBS</title>
<idno type="ISSN">0014-5793</idno>
<imprint><publisher>ELSEVIER</publisher>
<date type="published" when="1999">1999</date>
<biblScope unit="volume">463</biblScope>
<biblScope unit="issue">3</biblScope>
<biblScope unit="page" from="255">255</biblScope>
<biblScope unit="page" to="259">259</biblScope>
</imprint>
<idno type="ISSN">0014-5793</idno>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><idno type="ISSN">0014-5793</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cryoelectron Microscopy</term>
<term>Hemagglutinins, Viral (chemistry)</term>
<term>Hemagglutinins, Viral (ultrastructure)</term>
<term>Hydrogen-Ion Concentration</term>
<term>Influenza A virus (chemistry)</term>
<term>Models, Molecular</term>
<term>Protein Conformation</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Concentration en ions d'hydrogène</term>
<term>Conformation des protéines</term>
<term>Cryomicroscopie électronique</term>
<term>Hémagglutinines virales ()</term>
<term>Hémagglutinines virales (ultrastructure)</term>
<term>Modèles moléculaires</term>
<term>Virus de la grippe A ()</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Hemagglutinins, Viral</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="ultrastructure" xml:lang="en"><term>Hemagglutinins, Viral</term>
</keywords>
<keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Influenza A virus</term>
</keywords>
<keywords scheme="MESH" qualifier="ultrastructure" xml:lang="fr"><term>Hémagglutinines virales</term>
</keywords>
<keywords scheme="Teeft" xml:lang="en"><term>Acidic</term>
<term>Angular approach</term>
<term>Angular reconstitution</term>
<term>Biol</term>
<term>Cell biol</term>
<term>Central cavity</term>
<term>Cold spring harb</term>
<term>Conformation</term>
<term>Conformational</term>
<term>Conformational change</term>
<term>Conformational states</term>
<term>Conformational transitions</term>
<term>Cryoelectron Microscopy</term>
<term>Crystallographic structure</term>
<term>Digitised micrographs</term>
<term>Distal domains</term>
<term>Ectodomain</term>
<term>Entire ectodomain</term>
<term>Feb</term>
<term>Fourier shell correlation</term>
<term>Fourier space</term>
<term>Fusion capacity</term>
<term>Fusion peptide</term>
<term>Fusion sequence</term>
<term>Geel zwart</term>
<term>Haemagglutinin</term>
<term>Haemagglutinin trimer</term>
<term>Hydrogen-Ion Concentration</term>
<term>Image processing procedure</term>
<term>Individual trimers</term>
<term>Loop region</term>
<term>Membrane</term>
<term>Micrographs</term>
<term>Models, Molecular</term>
<term>Projection images</term>
<term>Protein Conformation</term>
<term>Rosette</term>
<term>Skehel</term>
<term>Subunit</term>
<term>Target membrane</term>
<term>Target membranes</term>
<term>Trimer</term>
<term>Trimeric ectodomain</term>
<term>Viral</term>
<term>Viral membrane</term>
<term>Whole trimer</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Concentration en ions d'hydrogène</term>
<term>Conformation des protéines</term>
<term>Cryomicroscopie électronique</term>
<term>Hémagglutinines virales</term>
<term>Modèles moléculaires</term>
<term>Virus de la grippe A</term>
</keywords>
</textClass>
<langUsage><language ident="en">en</language>
</langUsage>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Abstract: The three-dimensional structures of the complete haemagglutinin (HA) of influenza virus A/Japan/305/57 (H2N2) in its native (neutral pH) and membrane fusion-competent (low pH) form by electron cryo-microscopy at a resolution of 10 Å and 14 Å, respectively, have been determined. In the fusion-competent form the subunits remain closely associated preserving typical overall features of the trimeric ectodomain at neutral pH. Rearrangements of the tertiary structure in the distal and the stem parts are associated with the formation of a central cavity through the entire ectodomain. We suggest that the cavity is essential for relocation of the so-called fusion sequence of HA towards the target membrane.</div>
</front>
</TEI>
<affiliations><list><country><li>Allemagne</li>
<li>Royaume-Uni</li>
</country>
<region><li>Berlin</li>
<li>District de Giessen</li>
<li>Hesse (Land)</li>
</region>
<settlement><li>Berlin</li>
<li>Marbourg</li>
</settlement>
</list>
<tree><country name="Allemagne"><region name="Berlin"><name sortKey="Bottcher, Christoph" sort="Bottcher, Christoph" uniqKey="Bottcher C" first="Christoph" last="Böttcher">Christoph Böttcher</name>
</region>
<name sortKey="Herrmann, Andreas" sort="Herrmann, Andreas" uniqKey="Herrmann A" first="Andreas" last="Herrmann">Andreas Herrmann</name>
<name sortKey="Ludwig, Kai" sort="Ludwig, Kai" uniqKey="Ludwig K" first="Kai" last="Ludwig">Kai Ludwig</name>
<name sortKey="Stark, Holger" sort="Stark, Holger" uniqKey="Stark H" first="Holger" last="Stark">Holger Stark</name>
<name sortKey="Stark, Holger" sort="Stark, Holger" uniqKey="Stark H" first="Holger" last="Stark">Holger Stark</name>
<name sortKey="Stark, Holger" sort="Stark, Holger" uniqKey="Stark H" first="Holger" last="Stark">Holger Stark</name>
</country>
<country name="Royaume-Uni"><noRegion><name sortKey="Van Heel, Marin" sort="Van Heel, Marin" uniqKey="Van Heel M" first="Marin" last="Van Heel">Marin Van Heel</name>
</noRegion>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Sante/explor/H2N2V1/Data/Main/Exploration

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001987 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001987 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Sante
   |area=    H2N2V1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     ISTEX:2E44183DE2006E8235FC42EB2D04C37014438D4A
   |texte=   Structure of influenza haemagglutinin at neutral and at fusogenic pH by electron cryo-microscopy
}}

This area was generated with Dilib version V0.6.33.
Data generation: Tue Apr 14 19:59:40 2020. Site generation: Thu Mar 25 15:38:26 2021

Serveur d'exploration H2N2

Structure of influenza haemagglutinin at neutral and at fusogenic pH by electron cryo-microscopy

Structure of influenza haemagglutinin at neutral and at fusogenic pH by electron cryo-microscopy

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

	Serveur d'exploration H2N2
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.